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KMID : 0603820170230040310
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Journal of Experimental & Biomedical Science
2017 Volume.23 No. 4 p.310 ~ p.320
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Inhibitory Effects of Total Saponin Korean Red Ginseng on Thromboxane A₂ Production and P-Selectin Expression via Suppressing Mitogen-Activated Protein Kinases
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Shin Jung-Hae
Kwon Hyuk-Woo
Rhee Man-Hee
Park Hwa-Jin
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Abstract
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Ginseng has been widely used for traditional medicine in eastern Asia and is known to have inhibitory effects on cardiovascular disease (CVD) such as thrombosis, atherosclerosis, and myocardial infarction. Because, platelet is a crucial mediator of CVD, many studies are focusing on inhibitory mechanism of platelet functions. Among platelet activating molecules, thromboxane A₂ (TXA₂) and P-selectin play a central role in CVD. TXA₂ leads to intracellular signaling cascades and P-selectin plays an important role in platelet-neutrophil and platelet-monocyte interactions leading to the inflammatory response. In this study, we investigated the inhibitory mechanisms of total saponin fraction from Korean red ginseng (KRG-TS) on TXA₂ production and P-selectin expression. Thrombin-elevated TXA₂ production and arachidonic acid release were decreased by KRG-TS dose (25 to 150 μg/mL)-dependently via down regulation of microsomal cyclooxygenase-1 (COX-1), TXA₂ synthase (TXAS) activity and dephosphorylation of cytosolic phospholipase A₂ (cPLA₂). In addition, KRG-TS suppressed thrombin-activated P-selectin expression, an indicator of granule release via dephosphorylation of mitogen-activated protein kinases (MAPK). Taken together, we revealed that KRG-TS is a beneficial novel compound inhibiting TXA₂ production and P-selectin expression, which may prevent platelet aggregation-mediated thrombotic disease.
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KEYWORD
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Total saponin fraction, MAPKs, Thromboxane A₂, Arachidonic acid, P-selectin
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